Theuns et al. (Caries Res, 1993): data on mineral volume and birefringence of artificial enamel caries

   Theuns et al (Relationships between birefringence and mineral content in artificial caries lesion of enamel. Caries Research, 27:9-14, 1993) published data on the mineral volume and birefringence in water of artificial enamel caries that can be used to derive water and organic volumes. Data on the surface layer are of particular importance because they can be used to quantify the amount of organic volume in the outer layer of enamel caries close the acidic gel used to create the artificial caries lesion. If organic matter is incorporated into enamel during cariogenic challenge with gels in vitro, permeability and optical properties might be altered significantly compared to situations where no organic content is available for incorporation. The quantity of organic matter can be compared with that commonly found in normal enamel (~2%). Using equations for interpretation of enamel birefringence from Sousa et al. (J Microsc, 2006), anyone can test this.
  Numerical data, extracted from graphs in the article using image analysis software, follow below (Vmin = mineral volume based on a mineral density of 2.99 gm-3; BRwater =birefringence in water with sign, positive or negative; total of 72 lines):

Vmin        BRwater (x 10-4)

33.56 24.07
49.126 9.63
49.832 32.18
49.832 47.4
52.659 -0.37
54.072 21.11
54.425 22.96
55.132 22.59
55.309 24.255
58.321 26.105
58.675 26.475
59.028 24.07
59.028 -0.37
59.028 27.955
62.384 30.74
63.268 10.74
64.681 9.075
64.858 1.11
65.388 29.63
65.388 20.925
65.388 -0.37
65.741 17.5
66.801 20.925
67.154 22.035
68.224 12.18
68.224 12.775
68.224 5.735
68.224 3.7
68.224 -7.965
68.997 9.26
69.814 37.585
71.05 12.775
71.05 -0.185
71.227 44.44
71.227 23.145
71.58 16.29
72.994 3.515
72.994 5.735
72.994 -7.965
73.347 4.44
74.054 10.185
74.054 17.215
74.76 -4.255
74.937 17.77
75.997 13.33
76.88 12.22
76.88 -0.555
77.235 24.995
77.596 -5.005
77.596 -7.595
78.126 -10.925
78.126 6.29
78.656 -8.705
78.709 8.695
79.539 -16.66
79.893 -10.37
79.893 -0.37
80.599 -5.56
80.599 -19.63
80.776 -19.815
82.366 -17.225
82.719 -12.045
82.896 7.77
83.779 -0.555
83.779 -23.33
84.133 -10.74
84.133 -12.775
84.133 -3.7
84.663 -3.885
84.663 -10.555
86.429 -20.925
86.429 -2.59
 

   As an example, line 2 results in an organic volume of 31.547 % and a total water volume of 19.327 %. Summed with mineral volume of 49.126% yields 100%, for numerical validation.